ABSTRACT
A total of 2,605 faecal specimens from children up to 10 years old with or without diarrhoea were collected. Samples were obtained from 1986 to 2000 in hospitals, outpatient clinics and day-care centers in Goiânia, Goiás. Two methodologies for viral detection were utilized: a combined enzyme immunoassay for rotavirus and adenovirus and polyacrylamide gel electrophoresis. Results showed 374 (14.4 percent) faecal specimens positive for Rotavirus A, most of them collected from hospitalized children. A significant detection rate of rotavirus during the period from April to August, dry season in Goiânia, and different frequencies of viral detection throughout the years of study were also observed. Rotavirus was significantly related to hospitalization and to diarrhoeal illness in children up to 24 months old. This study reinforces the importance of rotavirus as a cause of diarrhoea in children and may be important in regards to the implementation of rotavirus vaccination strategies in our country
Subject(s)
Humans , Male , Female , Infant, Newborn , Infant , Child, Preschool , Child , Diarrhea , Rotavirus , Rotavirus Infections , Brazil , Chi-Square Distribution , Diarrhea , Electrophoresis, Polyacrylamide Gel , Feces , Immunoenzyme Techniques , Prevalence , Rotavirus Infections , SeasonsABSTRACT
The presence of serological markers for hepatitis B virus (HBsAg, anti-HBc IgM and Anti-HBc total) was investigated in the serum of 1,396 individuals who had clinical suspect of hepatitis. It was observed that 50.7 percent of the individuals were positive and, from the total of the studied individuals, 14.5 percent were positive for HBsAg. From these, 8.5 percent were also positive for anti-HBc IgM. The analysis in relation to gender showed a higher seroprevalence index among male individuals (p < 0.0001). It was observed the occurrence of subtypes adw2 (62.7 percent), ayw3 (23.5 percent), ayw2 (9.8 percent) and adw4 (3.9 percent). The viral DNA was detected in 61 (33.9 percent) HBsAg positive samples and in one sample positive only for anti-HBc total. These results indicate an important incidence of the HBV infection in this population, and reinforce previous studies regarding this virus in the central west region of Brazil
Subject(s)
Humans , Male , Female , DNA, Viral , Hepatitis B , Hepatitis B virus , Biomarkers , Brazil , Hepatitis B , Hepatitis B Antibodies , Hepatitis B Antigens , Hepatitis B virus , Seroepidemiologic Studies , SerotypingABSTRACT
Análise sorológica visando detecçåo de anticorpos para o vírus da rubéola foi feita em 1025 indivíduos da cidade de Goiânia - Goiás, no período de 1982 a 1990. A faixa etária variou de < 1 mês a 74 anos de idade. Observou-se uma soroprevalência de 86,5 pôr cento através da técnica de Inibiçåo da Hemaglutinaçåo, tomando como padråo de soropositividade o título maior ou igual a 1:16. A soroprevalência aumentou com a idade para indivíduos maiores que 6 anos